Title page for ETD etd-12232009-114026


Type of Document Master's Thesis
Author Madsen, Sara Lindsly
URN etd-12232009-114026
Title Gene Expression of the Intestinal Peptide Transporter 1 and Peroxisome Proliferator-Activated Receptor alpha Following Fasting/Refeeding and Ligand Administration in Chickens
Degree Master of Science
Department Animal and Poultry Sciences
Advisory Committee
Advisor Name Title
Wong, Eric A. Committee Chair
Denbow, Donald Michael Committee Member
McElroy, Audrey P. Committee Member
Keywords
  • PepT1
  • Fasting
  • Chicken
  • PPARα
Date of Defense 2009-12-11
Availability unrestricted
Abstract
The uptake of amino acids is mediated by amino acid transporters and the peptide transporter, PepT1. The nuclear receptor PPARα may play a role in the activation of PepT1. The objectives of this study were to evaluate the effect of a fasting/refeeding regimen and gavaging a PPARα ligand on expression of chicken PepT1 and PPARα. During the fasting/refeeding trial, chicks were placed on a 24h fast and then divided into 3 groups (Continuously Fasted (CF), Refed/Food Withdrawn, and Refed Adlib) and sampled 1, 2, 3, 5 and 7h post-fast. Expression of PepT1 and PPARα increased almost 2-fold post-24h fast (P < 0.002). The CF group had highest expression for both genes (P < 0.0001) suggesting a coordinate change in PepT1 and PPARα expression during fasting, indicating a possible regulatory role for PPARα of PepT1. Oral gavage trials utilizing PPARα ligand WY- 14643 were conducted with layers and broilers. For the layer trial birds were gavaged daily for 3 d with 50 mg WY-14643/kg in carrier (Ligand) or with just carrier (No Ligand) and sampled on d4. For the broiler trial, birds were gavaged daily with 25 or 50 mg WY-14643/kg or just carrier and sampled on d2 or d3 post gavage. No difference in PepT1 was observed between the Ligand and No Ligand groups. In the layer trial, PPARα decreased 6-fold (P = 0.005) in the Ligand group. Broilers treated with WY-14643 showed no effect of ligand. These results indicate a difference between layers and broilers in response to WY-14643.
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