Type of Document Master's Thesis Author McCollum, Martha Quinn URN etd-1240132199612941 Title Lysine and Glycyl-L-Sarcosine Absorption Across Ovine Forestomach Epithelium In Vitro Degree Master of Science Department Animal and Poultry Sciences Advisory Committee
Advisor Name Title Herbein, Joseph H. Jr. Kornegay, Ervin T. Webb, Kenneth E. Jr. Committee Chair Keywords
- amino acid
Date of Defense 1996-08-20 Availability unrestricted Abstract
Lysine absorption by ruminal and omasal
epithelia was studied using parabiotic
chambers that were sampled for 60-min.
Lysine appearance in serosal buffers and the
accumulation of lysine in tissues increased
linearly (P < .001) with time. Lysine
appearance in serosal buffers of ruminal tissue
increased proportionally as the concentration
of lysine increased in mucosal buffers.
However, lysine appearance in serosal buffers
of omasal tissue increased proportionally to a
substrate concentration of 1.5 mM, then
plateaued. Total absorption (tissue
accumulation plus serosal appearance)
increased linearly for ruminal tissue; however,
for omasal tissue, total absorption increased
linearly to 1.5 mM (P < .001), then plateaued.
Using omasal epithelium, glycyl-L-sarcosine
(Gly-Sar; .1 mM) absorption was studied
during co-incubation with glycine and peptide
substrates (each at 5 mM). Accumulation of
Gly-Sar in omasal epithelium was greatest (P <
.05) when Gly-Sar was present alone. Glycine
inhibited (P < .05) Gly-Sar accumulation by
20%, whereas peptide substrates inhibited (P
< .05) Gly-Sar accumulation by 60 to 85%.
The absorption of Gly-Sar (.1 mM) alone or
during co-incubation with either 10 mM butyric
acid, or a mixture of VFA was also studied.
Accumulation of Gly-Sar in tissue was greatest
(P < .05) when Gly-Sar was present alone;
butyric acid and VFA inhibited (P < .05)
Gly-Sar accumulation by 50 to 84%. These
results suggest absorption of amino acids and
peptides by the omasum, and also suggest the
mechanism involves mediated as well as
possibly paracellular transport.
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