Type of Document Major Paper Author Elhadj, Selim Author's Email Address elhadj@vt.edu URN etd-41298-161018 Title Economic Feasibility of the Recovery of Therapeutic Proteins From the Milk of Transgenic Livestock Degree Master of Engineering Department Chemical Engineering Advisory Committee
Advisor Name Title Davis, Richey M. Williams, Kimberly Forsten Velander, William H. Committee Chair Keywords
- human serum albumin
- human protein C
- factor VIII
- therapeutic protein
- manufacturing cost
- recovery
- milk
- transgenic
Date of Defense 1998-05-05 Availability unrestricted Abstract The recovery of pharmaceuticals from blood plasma is often expensive, limited,
and is subject to contamination. The objective of this feasibility study is to
present recovery designs and the most significant parameters affecting
manufacturing costs of human Serum Albumin (hSA), human Protein C (hPC), and
Factor VIII (FVIII) from the milk of transgenic livestock. All the processes
presented are based on chromatography columns to achieve separation. The
sensitivity of the manufacturing cost of the protein to various parameters
are illustrated graphically and discussed, along with the cost distribution
percentages for each type of process. It was found that manufacturing costs
decrease with superficial velocity, expression levels, column yields,
column capacities, and scale of production increases with all other parameters
remaining constant. However the estimated cost decreases tend to level out at
higher values of the parameters. Finally a judicious choice of
separation scheme, such as Fall-Through (FT) versus Direct Adsorption (DA) for
hSA production, can decrease the protein cost by as much as 38%, due to savings
from wash and elution buffers. The costs associated with the production of hSA
(DA process), hSA (FT process), hPC, and FVIII are 3.56, 2.20, 129, and 11,780
$/gr respectively. By means of the optimization of manufacturing parameters to
reduce costs, key areas of emphasis for process development were identified.
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