Title page for ETD etd-71297-151953


Type of Document Master's Thesis
Author Perkins, Talayia Nayette
Author's Email Address jhwms@vt.edu
URN etd-71297-151953
Title The effect of reactive oxygen species on aged skeletal muscle
Degree Master of Science
Department Human Nutrition, Foods, and Exercise
Advisory Committee
Advisor Name Title
Moore, David Michael
Thomas, Elizabeth A.
Williams, Jay H. Committee Chair
Keywords
  • calcium ATPase
  • sarcoplasmic reticulum
  • aging
Date of Defense 1997-07-30
Availability restricted
Abstract
The production of reactive oxygen species (ROS) may be a contributor to the progression of sarcopenia. Sarcopenia is a generic term for the loss of skeletal muscle mass, quality and strength. ROS are usually produced by radiation, but are also the byproducts of aerobic metabolism. ROS have been found to mediate various pathological conditions in a variety of tissues, to cause oxidative damage to DNA, proteins, and lipids with advancing age, and is presumably a major factor contributing to changes associated with aging. The purpose of this investigation was to determine whether the sarcoplasmic reticulum (SR) of muscle from aged animals are more susceptible to the deleterious effects of ROS. Using isolated gastrocnemius SR vesicles extracted from adult (12m) and aged (27m) male Brown Norway-Fischer 344 hybrid rats, Ca2+ uptake and release measurements were obtained. The data showed that there was a 33% difference between aged and adult gastrocnemius mass. When gastrocnemius mass was corrected for body mass, the differences was ~20% between the two groups. A 20% decrease in SR Ca2+ uptake rate was noted in aged animals. HOCl also, decreased uptake by similar extents in both groups. This result suggest that the Ca2+ pump's response to ROS are similar in both groups. AgNO3 -induced and H2O2 -induced release in aged animals was 17.94 and 7.39 nmol/mg/min and in adult animals was 30.46 and 7.18 nmol/mg/min, respectively. H2O2-induced release, when expressed as a percent of AgNO3-induced release was increased in aged animals by 54%. The results suggest that the release channel of aged muscle appears to be more sensitive to ROS. In conclusion, the data support the theory that aged animal skeletal muscle is more susceptible to the adverse effects of ROS.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
[VT] APENDIXA.PDF 44.76 Kb 00:00:12 00:00:06 00:00:05 00:00:02 < 00:00:01
[VT] CHAP1.PDF 53.37 Kb 00:00:14 00:00:07 00:00:06 00:00:03 < 00:00:01
[VT] CHAP3.PDF 31.55 Kb 00:00:08 00:00:04 00:00:03 00:00:01 < 00:00:01
[VT] CHAP4.PDF 43.39 Kb 00:00:12 00:00:06 00:00:05 00:00:02 < 00:00:01
[VT] CHAP5.PDF 29.77 Kb 00:00:08 00:00:04 00:00:03 00:00:01 < 00:00:01
[VT] PRE1.PDF 35.85 Kb 00:00:09 00:00:05 00:00:04 00:00:02 < 00:00:01
[VT] PRE2.PDF 33.12 Kb 00:00:09 00:00:04 00:00:04 00:00:02 < 00:00:01
[VT] REFER.PDF 62.98 Kb 00:00:17 00:00:08 00:00:07 00:00:03 < 00:00:01
[VT] REVLITER.PDF 34.12 Kb 00:00:09 00:00:04 00:00:04 00:00:02 < 00:00:01
[VT] VITA.PDF 17.05 Kb 00:00:04 00:00:02 00:00:02 00:00:01 < 00:00:01
[VT] indicates that a file or directory is accessible from the Virginia Tech campus network only.

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.