Title page for ETD etd-71497-17302


Type of Document Master's Thesis
Author Lo, Terry
Author's Email Address tlo
URN etd-71497-17302
Title Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Sriranganathan, Nammalwar
Wong, Eric A.
Inzana, Thomas J. Committee Chair
Keywords
  • PCR
  • multiplex
  • serotyping
  • Actinobacillus pleuropneumoniae
Date of Defense 1997-07-31
Availability unrestricted
Abstract
Traditional serologic assays of Actinobacillus pleuropeumoniae often have

problems with cross-reactivity. To avoid the complications of antibody-antigen

reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae

and identify serotype 5 strains. Primers specific to the conserved capsular export

region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the

exception of serotype 4. A second set of primers specific to the unique capsular

biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5

only. The sensitivity of this assay was determined to be less than 100 colony

forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively

distinguishes serotype 5 strains from other serotyes.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
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  At.pdf 572.13 Kb 00:02:38 00:01:21 00:01:11 00:00:35 00:00:03
  vita.pdf 4.99 Kb 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01

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