Title page for ETD etd-71497-17302

Type of Document

Master's Thesis

Author

Lo, Terry

Author's Email Address

tlo

URN

etd-71497-17302

Title

Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction

Degree

Master of Science

Department

Veterinary Medical Sciences

Advisory Committee

Advisor Name	Title
Dr. Inzana	Committee Chair
Eric A. Wong	none
Nammalwar Sriranganathan	none

Keywords

PCR
multiplex
serotyping
Actinobacillus pleuropneumoniae

Date of Defense

1997-07-31

Availability

unrestricted

Abstract

Traditional serologic assays of Actinobacillus pleuropeumoniae often have
problems with cross-reactivity. To avoid the complications of antibody-antigen
reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae
and identify serotype 5 strains. Primers specific to the conserved capsular export
region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the
exception of serotype 4. A second set of primers specific to the unique capsular
biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5
only. The sensitivity of this assay was determined to be less than 100 colony
forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively
distinguishes serotype 5 strains from other serotyes.

Files

Filename		Size	Approximate Download Time (Hours:Minutes:Seconds)
Filename		Size	28.8 Modem	56K Modem	ISDN (64 Kb)	ISDN (128 Kb)	Higher-speed Access
	At.pdf	572.13 Kb	00:02:38	00:01:21	00:01:11	00:00:35	00:00:03
	vita.pdf	4.99 Kb	00:00:01	< 00:00:01	< 00:00:01	< 00:00:01	< 00:00:01