Title page for ETD etd-81097-11925


Type of Document Master's Thesis
Author Bowers, Sharon H.
Author's Email Address webbk@vt.edu
URN etd-81097-11925
Title Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles
Degree Master of Science
Department Animal and Poultry Sciences
Advisory Committee
Advisor Name Title
Webb, Kenneth E. Jr. Committee Chair
Herbein, Joseph H. Jr. Committee Member
Kornegay, Ervin T. Committee Member
Keywords
  • Sheep
  • Jejunum
  • Ileum
  • Peptide
  • Absorption
  • Transport
Date of Defense 1997-08-19
Availability unrestricted
Abstract
In order to characterize peptide transport in the ovine small intestine, [14C]-glycyl-sarcosine uptake by tissue collected from five sheep was studied through the use of brush border membrane vesicles (BBMV). Preliminary experiments determined that incubation in hyaluronidase is not necessary in order to separate mucosal tissue from the basement membrane and that the stop solution used in the uptake study needed to be buffered. Uptake was examined in proximal (denoted jejunal) and distal (denoted ileal) halves of the intestine at four times (15, 30, 45, and 60 s) and at three extravesicular pH levels (6.4, 7.0, and 7.5). An intravesicular pH of 7.5 was used throughout the study. The two tissue sites differed (P < .02), with BBMV from jejunal tissue showing greater uptake than ileal. Uptake plateaued after 45 s, resulting in a quadratic (P < .005) effect of time. The effect of changes in extravesicular pH was also quadratic (P < .04), with uptake being greatest at pH 6.4, lowest at pH 7.0 and intermediate between the two at pH 7.5. Peptide uptake by sheep jejunal and ileal BBMV was demonstrated, but there was no clear evidence for increased uptake with decreasing extravesicular pH.
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