Title page for ETD etd-8598-184718


Type of Document Master's Thesis
Author Cutright, Rebecca J.
Author's Email Address rjcutrig@unity.ncsu.edu
URN etd-8598-184718
Title Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families
Degree Master of Science
Department Horticulture
Advisory Committee
Advisor Name Title
Veilleux, Richard E. Committee Chair
Beers, Eric P. Committee Member
Griffey, Carl A. Committee Member
Keywords
  • Potato
  • Anther Culture
  • Haploid-inducing Pollinator
Date of Defense 1998-07-16
Availability unrestricted
Abstract
Monopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 x IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones.
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  ch1.pdf 1.11 Mb 00:05:08 00:02:38 00:02:18 00:01:09 00:00:05
  ch1_figs.pdf 64.38 Kb 00:00:17 00:00:09 00:00:08 00:00:04 < 00:00:01
  ch2.pdf 71.55 Kb 00:00:19 00:00:10 00:00:08 00:00:04 < 00:00:01
  ch2_figs.pdf 293.04 Kb 00:01:21 00:00:41 00:00:36 00:00:18 00:00:01
  ch2_tbls.pdf 72.67 Kb 00:00:20 00:00:10 00:00:09 00:00:04 < 00:00:01
  ch3.pdf 71.00 Kb 00:00:19 00:00:10 00:00:08 00:00:04 < 00:00:01
  ch3_figs.pdf 167.33 Kb 00:00:46 00:00:23 00:00:20 00:00:10 < 00:00:01
  ch3_tbls.pdf 38.13 Kb 00:00:10 00:00:05 00:00:04 00:00:02 < 00:00:01
  intro.pdf 44.78 Kb 00:00:12 00:00:06 00:00:05 00:00:02 < 00:00:01
  vita.pdf 213.71 Kb 00:00:59 00:00:30 00:00:26 00:00:13 00:00:01

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