Scholarly
    Communications Project


Document Type:Master's Thesis
Name:Tang Youlin
Email address:yotang@vt.edu
URN:1998/00633
Title:Response of Leaf Protein to Ozone in Two White Clover Clones
Degree:Master of Science
Department:Plant Pathology, Physiology and Weed Science
Committee Chair: Boris I. Chevone and John L. Hess
Chair's email:bchevone@vt.edu and jlhess@vt.edu
Committee Members:Boris I. Chevone
John L. Hess
Laurence D. Moore
Keywords:White Clover, Ozone-Responsive Protein
Date of defense:May 7, 1998
Availability:Release the entire work for Virginia Tech access only.
After one year release worldwide only with written permission of the student and the advisory committee chair.

Abstract:

Response of Leaf Protein to Ozone in White Clover Clones Youlin Tang (Abstract) A white clover ( Trifolium repens ) system, based on one ozone-sensitive (NC-S) and one ozone-resistant (NC-R) clone, has been developed as an indicator to estimate the effects of tropospheric ozone on plant biomass production. A reduction in the vegetative biomass ratio (NC-S/NC-R) of the clones was correlated with increasing concentrations of ozone during a 28-day exposure period. However, the mechanism of ozone sensitivity or tolerance at the biochemical or molecular level is not known. Superoxide dismutase isozyme activities in the two clones did not respond differently to ozone treatment. However, catalase activity increased somewhat more in the leaf tissue of NC-R, compared to NC-S, after ozone treatment. Two-dimensional polyacrylamide gel electrophoresis (PAGE) showed the presence of two proteins (Ozone-Response-Proteins, ORPs) that were more responsive to ozone in the tolerant genotype than in the sensitive one. After ozone treatment for three days, the ORPs were four-fold higher in leaf tissue of NC-R compared to NC-S. Also, the amount of the ORPs was twenty-fold higher in leaf tissue of ozone-treated NC-R than in that of control NC-R. These proteins have apparent molecular weights of 21.5 kD and 23 kD and isoelectric points of 4.1-4.4 on SDS-PAGE gels. The filtrate of a 100 kD concentrator showed that the native molecular weights of the ORPs were less than 100 kD. The results obtained from a study of field samples demonstrated that protein content in leaf tissue of both NC-R and NC-S was positively correlated with ORP content.

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Dissertation-1.pdf

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