Scholarly Communications Project

Document Type:Master's Thesis
Name:Terry Lo
Email address:tlo
URN:1997/00093
Title:Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
Degree:Master of Science
Department:VMS
Committee Chair: Dr. Inzana
Chair's email:tinzana@vt.edu
Committee\ Members:
Keywords:PCR, multiplex, serotyping, Actinobacillus pleuropneumoniae
Date of defense:July 31, 1997
Availability:Release the entire work for Virginia Tech access only.
After one year release worldwide only with written permission of the student and the advisory committee chair.

Abstract:

Traditional serologic assays of Actinobacillus pleuropeumoniae often have problems with cross-reactivity. To avoid the complications of antibody-antigen reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. Primers specific to the conserved capsular export region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the exception of serotype 4. A second set of primers specific to the unique capsular biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5 only. The sensitivity of this assay was determined to be less than 100 colony forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively distinguishes serotype 5 strains from other serotyes.

List of Attached Files

At.pdf vita.pdf

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