Scholarly
    Communications Project


Document Type:Master's Thesis
Name:Simge Baloglu
Email address:sbaloglu@vt.edu
URN:1997/00135
Title:ASSESSMENT OF THE EXPRESSION OF BRUCELLA ABORTUS HEAT SHOCK PROTEIN, GROEL, IN VACCINIA VIRUS TO INDUCE PROTECTION AGAINST A BRUCELLA CHALLENGE IN BALB/C MICE
Degree:Master Of Science
Department:Veterinary Medical Sciences
Committee Chair: Stephen M. Boyle
Chair's email:smboyle@vt.edu
Committee Members:
Keywords: Brucellosis, Attenuated Live Vaccines, Chaperonins
Date of defense:July 8,1997
Availability:Release the entire work for Virginia Tech access only.
After one year release worldwide only with written permission of the student and the advisory committee chair.

Abstract:

B. abortus is an intracellular facultative bacterial pathogen which causes abortion in cattle and undulant fever in humans. Cattle vaccines such as B. abortus strains 19 and RB51 are live vaccine strains which protect approximately 75% of the vaccinated animals. No effective vaccines are available for the prevention of brucellosis in humans. We are developing vaccinia virus recombinants expressing various B. abortus proteins to prevent brucellosis in susceptible mammalian species. In this work the B. abortus groEL gene encoding the antigenic heat shock protein GroEL was subcloned into vaccinia virus via homologous recombination. Expression of the GroEL protein in vaccinia infected cells in-vivo was confirmed by immunoblotting. Groups of 5 female BALB/C mice were injected with the vaccinia recombinant or appropriate positive and negative control vaccines. Mice were bled and their humoral immune responses assessed. In addition, mice were challenged with virulent B. abortus strain 2308 and protection measured by the rate of splenic clearance of live Brucella. In spite of demonstrating specific GroEL antibodies in recombinant vaccinia injected mice, no significant level of protection was demonstrable. Preliminary lymphocyte transformation assays were carried out to establish if a cell mediated immune response to GroEL was induced in the vaccinated animals.

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